Bioluminizer® 2, Biochimie și Biotehnologii

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Bioluminizer® 2

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The CAMAG BioLuminizer® 2 is a detection system specifically designed to detect bioluminescence on HPTLC plates

The BioLuminizer® 2 system is consisting of a compartment excluding any extraneous light, climate controlled for extended stability of the plate, and a 16 bit CCD digital camera of high resolution and high quantum efficiency. It features ergonomic design and easy, intuitive handling in stand-alone mode using the special software. With the BioLuminizer® 2 bioactivity can be detected and registered economically and with short response time. Special antibacterial protection measures are not necessary, as the bioluminescent bacteria Allivibrio fischeri are non-pathogenic.

Hyphenating TLC/HPTLC and bioassay is an excellent tool for identification of single toxic compounds in complex sample matrices. The method is suitable for the detection of toxins in foodstuff, beverages, cosmetics, wastewater, drinking water, and for the detection of bioactivity in natural products. After chromatographic separation of the complex sample the plate is immersed in a suspension of bioluminescent bacteria Allivibrio fischeri. The reaction takes place within a very short time. All zones with inhibitory or toxic effects appear as dark zones on the luminescent plate background.

Key features
• Stand-alone operation using BioLuminizer® 2 Software
• Cooled 16 bit CCD camera with high resolution and high quantum efficiency
• Optimized plate compartment, which preserves a favorable environment for luminescent bacteria (the bacteria are kept moist)
• Small footprint
• Easy to clean

Example
Processed wastewater containing X-ray contrast media is frequently irradiated with UV light. The HPTLC bioluminescence image shows the bioactive effect of degradation products. As can be seen, an increase of the irradiation time generates substances with a distinctively inhibitory effect on the luminescent bacteria. In a cuvette test, this inhibitory effect would have been masked by degradation products.
The example is taken from an internship report at the "Zweckverband Landeswasserversorgung" in Langenau, Germany.